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http://inaoe.repositorioinstitucional.mx/jspui/handle/1009/179| Multimodal microscopy for rapid diagnosis of pancreatic cancer | |
| MARIA DEL CARMEN GRADOS LUYANDO | |
| JULIO CESAR RAMIREZ SAN JUAN | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Cancer Microscopy Dyes Fluorescence | |
| Multimodal confocal mosaic images scan image wide surgical margins (approximately 1 cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is to combine three confocal imaging modalities: acridine orange (AO) fluorescence for labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous reflectance (R) for marking collagen and keratin. Absorption contrast is achieved by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm (Eo fluorescence). Scattering contrast is achieved by the reflectance at either or both of the wavelengths. Superposition and false-coloring of the images from these three modalities mimics H&E, enabling detection of pancreatic cell carcinomas. The sum of images Eo + R is false-colored pink to mimic the appearance of eosin, while the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in H&E. In this study, mosaics of pancreatic cell carcinomas where processed. The results suggest that confocal mosaics could be used for pancreatic cancer diagnosis in the operating room, although the staining process with Eosin may be optimized as the reflectance mode. | |
| Instituto Nacional de Astrofísica, Óptica y Electrónica | |
| 2014-10 | |
| Tesis de maestría | |
| Inglés | |
| Estudiantes Investigadores Público en general | |
| Grados-Luyando M.C. | |
| ÓPTICA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Maestría en Óptica |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| GradosLMC.pdf | 20.37 MB | Adobe PDF | Visualizar/Abrir |