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http://inaoe.repositorioinstitucional.mx/jspui/handle/1009/650| Development of a colorimetric metabolic assay and initial testing of photodynamic therapy photosensitizers for sterilization in the food industry | |
| NICTÉ YAXAL ARENAS HEREDIA | |
| JULIO CESAR RAMIREZ SAN JUAN | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Photodynamic therapy Biomedical measurement Bacterial quantification Biophysics | |
| This thesis implements a colorimetric assay to measure bacterial concentrations to test
new photosensitizers. The colorimetric assay was developed from a single point fluorescence to a time kinetic resazurin assay suitable for bacterial concentration measurements.
Listeria monocytogenes were treated with Photodynamic therapy (PDT) as a method
to decrease or sterilize the bacterial concentrations in an in vitro sample. PDT test were
performed using Methylene Blue as reference photosensitizer, to test the effective behavior
of the kinetic resasurin assay. Further PDT tests were useful to determine the bacterial
sterilization that new photosensitizers are capable off, this photosensitizers were developed
by Karen J. Brewer at the Virginia Polytechnic Institute. Photosensitizers are Mix
Metal Supramolecular Complexes (MMSC) that differ from other drugs used in PDT due
to the photosensitization process, where oxygen is not the most important element. En esta tesis se emplea un ensayo colorimétrico para medir concentraciones bacterianas, usándose en las pruebas preliminares de fotosensibilizadores. El ensayo colorimétrico se desarrolló a partir de medidas estáticas en el tiempo para ser usado con bacterias, terminando como un ensayo con dinámica temporal. Listeria monocytogenes fue tratando con Terapia fotodinámica (PDT) con la intención de disminuir o esterilizar in vitro concentraciones de bacterias. | |
| Instituto Nacional de Astrofísica, Óptica y Electrónica | |
| 2011-02 | |
| Tesis de maestría | |
| Español | |
| Estudiantes Investigadores Público en general | |
| Arenas-Heredia N.Y. | |
| ÓPTICA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Maestría en Óptica |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| ArenasHNY.pdf | 1.03 MB | Adobe PDF | Visualizar/Abrir |